The European antibody network's practical guide to finding and validating suitable antibodies for research

G Roncador, P Engel, L Maestre, AP Anderson… - MAbs, 2016 - Taylor & Francis
antibody validation because of the adverse effect that poor … of antibodies to a single protein,
eg, c-FLIPCitation 12 or to … many researchers through the maze of antibody validation and …

Antibody validation in bioimaging applications based on endogenous expression of tagged proteins

M Skogs, C Stadler, R Schutten… - … of proteome research, 2017 - ACS Publications
… Some researchers suggest that the problem is best tackled by only using recombinant …
for the target protein as well as an anti-GFP antibody in order to detect even low-abundance …

[HTML][HTML] Although it's painful: The importance of stringent antibody validation

VA Meliopoulos, S Schultz-Cherry - PLoS Pathogens, 2018 - journals.plos.org
… with low-abundance proteins, for which the antibody in question can only detect high levels
… We would not have known there was a problem with antibody 1 if we had not used KO mice …

Current challenges of metastatic breast cancer

B Lim, GN Hortobagyi - Cancer and Metastasis Reviews, 2016 - Springer
… early detection and improvement in survival owing to advancements in biomedical research
… in stage IV breast cancer, and we finish by noting new research initiatives to improve poor

Nanoparticles-based drug delivery and gene therapy for breast cancer: Recent advancements and future challenges

Z Mirza, S Karim - Seminars in cancer biology, 2021 - Elsevier
… and suffer concerns of low bioavailability, low cellular uptake, … antibodies allows both in vitro
and ex vivo optical diagnosis, … by activating caspase-8 and c-FLIP, but due to the changes in …

Genomic signatures in B-cell lymphoma: How can these improve precision in diagnosis and inform prognosis?

J Iqbal, H Naushad, C Bi, J Yu, A Bouska, J Rohr… - Blood Reviews, 2016 - Elsevier
… efforts from the biomedical research community to improve … myc-simple cases (cases with
Ig-myc according to and low … , FAS mutations and c-FLIP overexpression compromise the …

[HTML][HTML] The pyrrolo-1, 5-benzoxazepine, PBOX-15, enhances TRAIL‑induced apoptosis by upregulation of DR5 and downregulation of core cell survival proteins in …

SM Nathwani, LM Greene, S Butini… - International …, 2016 - spandidos-publications.com
… Apoptotic defects are frequently associated with poor outcome … Cell surface expression of
the receptors was then detected by … PBOX-15 can downregulate c-FLIP, resulting in increased …

Identification of MYC as an antinecroptotic protein that stifles RIPK1–RIPK3 complex formation

D Seong, M Jeong, J Seo, JY Lee… - … of Sciences, 2020 - National Acad Sciences
… a very transient or weak interaction between the two proteins (… , cIAP-1/2, and c-FLIP proteins
were not affected by changes in … analysis has become a routine tool in biomedical research, …

[HTML][HTML] A microplate-based nonradioactive protein synthesis assay: application to TRAIL sensitization by protein synthesis inhibitors

CJ Henrich - PLoS One, 2016 - journals.plos.org
detection using anti-puromycin antibodies have been previously reported and well-validated. …
However, the low throughput nature of the applications described to date (cellular imaging, …

Morphometrical, morphological, and immunocytochemical characterization of a tool for cytotoxicity research: 3D cultures of breast cell lines grown in ultra-low …

F Malhão, AC Macedo, AA Ramos, E Rocha - Toxics, 2022 - mdpi.com
… After the primary antibody incubation and after each step of the detection system, slides …
We selected a panel of antibodies targeting different outputs for the MCAs’ ICC characterization. …